We understand that accidents do occur, and we have options for when they do. If the suspected tie recently occurred, we can perform a cytology of the vagina in order to help confirm the suspicion. Ultrasound can also be performed 30 days after the suspected tie to confirm pregnancy. If pregnant the options include: allow the bitch to carry the litter to term, perform a spay or interrupt the pregnancy with medical treatment. Medical treatment takes place day 26 to 28 days after breeding.